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Part:BBa_K2872881:Design

Designed by: Dina Altarawneh   Group: iGEM18_CMUQ   (2018-10-10)


Template DNA sequence coding for HBB rs334 mutation (A to T) in humans, PAM site added


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 178


Design Notes

Prefix and suffix sites included, PAM site added


Source

Genomic sequence in humans with HBB mutations coding for Sickle cell anaemia (Carriers or affected)

References